PF4 Antibody View larger

PF4 Antibody

ASA-B1514

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

platelet factor 4 Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PF4(85-101aa DLQAPLYKKIIKKLLES).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PF4

Protein

Platelet factor 4(PF-4)

Uniprot ID

P02776

Function

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

Tissue Specificity

Sub-cellular localization

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Aliases

C-X-C motif chemokine 4 antibody|Chemokine(C X C motif) ligand 4 antibody|Chemokine(CXC motif) ligand 4 antibody|CXCL 4 antibody|CXCL4 antibody|Iroplact antibody|MGC138298 antibody|Oncostatin A antibody|Oncostatin-A antibody|OncostatinA antibody|PF 4 antibody|PF-4 antibody|PF4 antibody|Platelet factor 4 antibody|PLF4_HUMAN antibody|SCYB 4 antibody|SCYB4 antibody|short form antibody|Small inducible cytokine subfamily member 4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PF4 antibody, ASA-B1514, Western blotting
Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng
Anti-PF4 antibody, ASA-B1514, IHC(P)
IHC(P): Human Lung Cancer Tissue

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