More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| peroxiredoxin 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PRDX5 |
Protein | Peroxiredoxin-5, mitochondrial |
Uniprot ID | P30044 |
Function | Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Isoform Mitochondrial: Mitochondrion. |
Sequence Similarities | Belongs to the peroxiredoxin 2 family. |
Aliases | ACR 1 antibody|ACR1 antibody|Alu co repressor 1 antibody|Alu co-repressor 1 antibody|Alu corepressor 1 antibody|antioxidant enzyme B166 antibody|AOEB166 antibody|B166 antibody| liver tissue 2D-page spot 71B antibody|MGC117264 antibody|MGC142283 antibody|MGC142285 antibody|mitochondrial antibody|Peroxiredoxin 5 mitochondrial antibody|Peroxiredoxin 5, mitochondrial antibody|Peroxiredoxin V antibody|Peroxiredoxin-5 antibody|Peroxiredoxin5 antibody|peroxisomal antioxidant enzyme antibody|PLP antibody|PMP 20 antibody|PMP20 antibody|PRDX 5 antibody|PRDX 6 antibody|PRDX5 antibody|PRDX5_HUMAN antibody|PRDX6 antibody|Prx V antibody|Prx-V antibody|PRXV antibody|SBBI10 antibody|thioredoxin peroxidase PMP20 antibody|Thioredoxin reductase antibody|TPx type VI antibodyACR 1 antibody|ACR1 antibody|Alu co repressor 1 antibody|Alu co-repressor 1 antibody|Alu corepressor 1 antibody|antioxidant enzyme B166 antibody|AOEB166 antibody|B166 antibody| liver tissue 2D-page spot 71B antibody|MGC117264 antibody|MGC142283 antibody|MGC142285 antibody|mitochondrial antibody|Peroxiredoxin 5 mitochondrial antibody|Peroxiredoxin 5, mitochondrial antibody|Peroxiredoxin V antibody|Peroxiredoxin-5 antibody|Peroxiredoxin5 antibody|peroxisomal antioxidant enzyme antibody|PLP antibody|PMP 20 antibody|PMP20 antibody|PRDX 5 antibody|PRDX 6 antibody|PRDX5 antibody|PRDX5_HUMAN antibody|PRDX6 antibody|Prx V antibody|Prx-V antibody|PRXV antibody|SBBI10 antibody|thioredoxin peroxidase PMP20 antibody|Thioredoxin reductase antibody|TPx type VI antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Peroxiredoxin 5 antibody, ASA-B1512, Western blotting
All lanes: Anti Peroxiredoxin 5 (ASA-B1512) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti Peroxiredoxin 5 (ASA-B1512) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
Anti- Peroxiredoxin 5 antibody, ASA-B1512, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Peroxiredoxin 5 antibody, ASA-B1512, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue