Peroxiredoxin 3 Antibody View larger

Peroxiredoxin 3 Antibody

ASA-B1506

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

peroxiredoxin 3 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 3(239-256aa TIKPSPAASKEYFQKVNQ), different from the related mouse and rat sequences by three amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PRDX3

Protein

Thioredoxin-dependent peroxide reductase, mitochondrial

Uniprot ID

P30048

Function

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Tissue Specificity

Sub-cellular localization

Mitochondrion.

Sequence Similarities

Belongs to the AhpC/TSA family.

Aliases

Antioxidant Protein 1 antibody|AOP 1 antibody|AOP-1 antibody|AOP1 antibody|HBC189 antibody|MER 5 antibody|MER5 antibody|MGC104387 antibody|MGC24293 antibody|mitochondrial antibody|Mitochondrial thioredoxin dependent peroxide reductase antibody|peroxiredoxin 3 antibody|PeroxiredoxinIII antibody|Peroxiredoxin-3 antibody|Peroxiredoxin3 antibody|PRDX 3 antibody|PRDX3 antibody|PRDX3_HUMAN antibody|PRO1748 antibody|Protein MER5 homolog antibody|PRX III antibody|PRXIII antibody|Prx-III antibody|PRX3 antibody|SP 22 antibody|SP-22 antibody|SP22 antibody|Thioredoxin dependent peroxide reductase mitochondrial antibody|Thioredoxin dependent peroxide reductase precursor antibody|Thioredoxin-dependent peroxide reductase antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Peroxiredoxin 3 antibody, ASA-B1506, Western blotting
All lanes: Anti Peroxiredoxin 3 (ASA-B1506) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: A549 Whole Cell Ly
Anti-Peroxiredoxin 3 antibody, ASA-B1506, ICC
ICC: A549 Cell
Anti-Peroxiredoxin 3 antibody, ASA-B1506, IHC(P)
IHC(P): Human Lung Cancer Tissue

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