More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| peroxiredoxin 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PRDX3 |
Protein | Thioredoxin-dependent peroxide reductase, mitochondrial |
Uniprot ID | P30048 |
Function | Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | Belongs to the AhpC/TSA family. |
Aliases | Antioxidant Protein 1 antibody|AOP 1 antibody|AOP-1 antibody|AOP1 antibody|HBC189 antibody|MER 5 antibody|MER5 antibody|MGC104387 antibody|MGC24293 antibody|mitochondrial antibody|Mitochondrial thioredoxin dependent peroxide reductase antibody|peroxiredoxin 3 antibody|Peroxiredoxin III antibody|Peroxiredoxin-3 antibody|Peroxiredoxin3 antibody|PRDX 3 antibody|PRDX3 antibody|PRDX3_HUMAN antibody|PRO1748 antibody|Protein MER5 homolog antibody|PRX III antibody|Prx-III antibody|PRX3 antibody|SP 22 antibody|SP-22 antibody|SP22 antibody|Thioredoxin dependent peroxide reductase mitochondrial antibody|Thioredoxin dependent peroxide reductase precursor antibody|Thioredoxin-dependent peroxide reductase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Peroxiredoxin 3 antibody, ASA-B1508, Western blotting
All lanes: Anti Peroxiredoxin 3 (ASA-B1508) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: U20S Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: RH35 Whole Cell Lysate at 40u
All lanes: Anti Peroxiredoxin 3 (ASA-B1508) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: U20S Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: RH35 Whole Cell Lysate at 40u
Anti- Peroxiredoxin 3 antibody, ASA-B1508,IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
