Peroxiredoxin 1  Antibody View larger

Peroxiredoxin 1 Antibody

ASA-B1504

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

peroxiredoxin 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1 (100-128aa MNIPLVSDPKRTIAQDYGVLKADEGISFR), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PRDX1

Protein

Peroxiredoxin-1

Uniprot ID

Q06830

Function

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

Tissue Specificity

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the AhpC/TSA family.

Aliases

Hbp23 antibody|Heme binding 23 kDa protein antibody|Macrophage 23 Kd stress protein antibody|Macrophage 23kDa stress protein antibody|Macrophase stress protein 22kDa antibody|Macrophase stress protein 23 kd antibody|MSP23 antibody|Natural killer cell enhancing factor A antibody|Natural killer cell-enhancing factor A antibody|Natural killer enhancing factor A antibody|NKEF A antibody|NKEF-A antibody|NkefA antibody|OSF3 antibody|Osteoblast specific factor 3 antibody|PAG antibody|Paga antibody|PAGB antibody|Peroxiredoxin-1 antibody|Prdx 1 antibody|Prdx1 antibody|PRDX1_HUMAN antibody|Proliferation associated gene A antibody|Proliferation associated protein PAG antibody|Proliferation-associated gene protein antibody|PrxI antibody|Tdpx 2 antibody|Tdpx2 antibody|TDX2 antibody|Thioredoxin dependent peroxide reductase 2 antibody|Thioredoxin peroxidase 2 antibody|Thioredoxin-dependent peroxide reductase 2 antibody|TPxA antibody|Trx dependent peroxide reductase 2 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Peroxiredoxin 1 antibody, ASA-B1504, Western blotting
All lanes: Anti Peroxiredoxin 1 (ASA-B1504) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: A549 Whole Cell Lysate at 40ug
Lane 9: RH35 Whole
Anti- Peroxiredoxin 1 antibody, ASA-B1504, IHC(P)
IHC(P): Mouse Brain Tissue
Anti- Peroxiredoxin 1 antibody, ASA-B1504, IHC(P)
IHC(P): Rat Brain Tissue

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