More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| phosphatidylethanolamine binding protein 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PEBP1 |
Protein | Phosphatidylethanolamine-binding protein 1(PEBP-1) |
Uniprot ID | P70296 |
Function | Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity). |
Tissue Specificity | HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | HCNP antibody|HCNPpp antibody|Hippocampal cholinergic neurostimulating peptide antibody|Neuropolypeptide h3 antibody|PEBP antibody|PEBP 1 antibody|PEBP-1 antibody|PEBP1 antibody|PEBP1_HUMAN antibody|Phosphatidylethanolamine binding protein antibody|Phosphatidylethanolamine binding protein 1 antibody|Prostatic binding protein antibody|Prostatic-binding protein antibody|R kip antibody|Raf kinase inhibitor protein antibody|Raf kinase inhibitory protein antibody|RKIP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PBP antibody, ASA-B1485, Western blotting
All lanes: Anti PBP (ASA-B1485) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti PBP (ASA-B1485) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti-PBP antibody, ASA-B1485, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
