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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| PDZ binding kinase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PBK |
Protein | Lymphokine-activated killer T-cell-originated protein kinase |
Uniprot ID | Q96KB5 |
Function | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. |
Tissue Specificity | Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
Aliases | Cancer/testis antigen 84 antibody|CT84 antibody|Epididymis luminal protein 164 antibody|FLJ14385 antibody|HEL164 antibody|Lymphokine activated killer T cell originated protein kinase antibody|Lymphokine-activated killer T-cell-originated protein kinase antibody|MAPKK like protein kinase antibody|MAPKK-like protein kinase antibody|Nori 3 antibody|Nori-3 antibody|Nori3 antibody|PBK antibody|PDZ binding kinase antibody|PDZ-binding kinase antibody|Serine/threonine protein kinase antibody|Spermatogenesis related protein kinase antibody|Spermatogenesis-related protein kinase antibody|SPK antibody|T LAK cell originated protein kinase antibody|T-LAK cell-originated protein kinase antibody|TOPK antibody|TOPK_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PBK antibody, ASA-B1484, Western blotting
All lanes: Anti PBK (ASA-B1484) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti PBK (ASA-B1484) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- PBK antibody, ASA-B1484, IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti- PBK antibody, ASA-B1484, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue