More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| poly (ADP-ribose) polymerase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PARP1 |
Protein | Poly [ADP-ribose] polymerase 1 |
Uniprot ID | P09874 |
Function | Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Nucleus, nucleolus. Note: Localizes at sites of DNA damage. |
Sequence Similarities | Contains 1 BRCT domain. |
Aliases | ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody|ADP ribosyltransferase diphtheria toxin like 1 antibody|ADP ribosyltransferase NAD(+) antibody|ADP ribosyltransferase NAD+ antibody|ADPRT 1 antibody|ADPRT antibody|ADPRT1 antibody|ARTD1 antibody|msPARP antibody|NAD(+) ADP ribosyltransferase 1 antibody|NAD(+) ADP-ribosyltransferase 1 antibody|pADPRT 1 antibody|pADPRT1 antibody|PARP 1 antibody|PARP antibody|PARP-1 antibody|PARP1 antibody|PARP1_HUMAN antibody|Poly (ADP ribose) polymerase 1 antibody|poly (ADP ribose) polymerase family, member 1 antibody|Poly [ADP-ribose]polymerase 1 antibody|Poly adenosine diphosphate ADP ribose polymerase antibody|Poly ADP ribose polymerase 1 antibody|Poly ADP ribose polymerase family member 1 antibody|Poly ADP ribose synthetase 1 antibody|Poly(ADP ribose) polymerase antibody|poly(ADP ribose) synthetase antibody|poly(ADP ribosyl)transferase antibody|Poly[ADP ribose] synthetase 1 antibody|Poly[ADP-ribose] synthase 1 antibody|PPOL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PARP antibody, ASA-B1478, Western blotting
All lanes: Anti PARP (ASA-B1478) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: PC12 Whole Cell Lysate at 40ug
Lane 7: NIH Whole Cell Lysate at 40ug
Predicted bind size: 113KD
Observed bind size: 150KD
All lanes: Anti PARP (ASA-B1478) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: PC12 Whole Cell Lysate at 40ug
Lane 7: NIH Whole Cell Lysate at 40ug
Predicted bind size: 113KD
Observed bind size: 150KD
Anti- PARP antibody, ASA-B1478, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- PARP antibody, ASA-B1478, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue