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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| parkinson protein 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PARK7 |
Protein | Protein DJ-1 |
Uniprot ID | Q99497 |
Function | Protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteines, arginines and lysines residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) (PubMed:25416785). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Its involvement in protein repair could also explain other unrelated functions. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). |
Tissue Specificity | Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the peptidase C56 family. |
Aliases | CAP1 antibody|DJ 1 antibody|DJ-1 antibody|DJ1 antibody|DJ1 protein antibody|FLJ27376 antibody|FLJ34360 antibody|FLJ92274 antibody|Oncogene DJ1 antibody|OTTHUMP00000001348 antibody|OTTHUMP00000001349 antibody|OTTHUMP00000001350 antibody|OTTHUMP00000001351 antibody|Park 7 antibody|Park7 antibody|PARK7_HUMAN antibody|Parkinson disease (autosomal recessive early onset) 7 antibody|Parkinson disease (autosomal recessive, early onset) 7 antibody|Parkinson disease protein 7 antibody|Parkinson protein 7 antibody|Protein DJ 1 antibody|Protein DJ-1 antibody|RNA binding protein regulatory subunit antibody|RS antibody|SP22 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PARK7 antibody, ASA-B1475, Western blotting
All lanes: Anti PARK7 (ASA-B1475) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 20KD
Observed bind size: 20KD
All lanes: Anti PARK7 (ASA-B1475) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 20KD
Observed bind size: 20KD
Anti- PARK7 antibody, ASA-B1475, IHC(P)
IHC(P): Mouse Pancreas Tissue
IHC(P): Mouse Pancreas Tissue
Anti- PARK7 antibody, ASA-B1475, IHC(P)
IHC(P): Rat Pancreas Tissue
IHC(P): Rat Pancreas Tissue