More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| parathyroid hormone 1 receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Parathyroid Hormone Receptor 1(388-406aa KLRETNAGRCDTRQQYRKL), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTH1R |
Protein | Parathyroid hormone/parathyroid hormone-related peptide receptor |
Uniprot ID | Q03431 |
Function | This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. |
Tissue Specificity | Expressed in most tissues. Most abundant in kidney, bone and liver. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | MGC138426 antibody|MGC138452 antibody|Parathyroid hormone 1 receptor antibody|Parathyroid hormone/parathyroid hormone related peptide receptor antibody|Parathyroid hormone/parathyroid hormone related protein receptor antibody|Parathyroid hormone/parathyroid hormone-related peptide receptor antibody|PTH receptor antibody|PTH/PTHr receptor antibody|PTH/PTHrP receptor antibody|PTH/PTHrP type I receptor antibody|PTH1 receptor antibody|PTH1R antibody|PTH1R_HUMAN antibody|PTHR 1 antibody|PTHR antibody|PTHR1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Parathyroid Hormone Receptor 1 antibody, ASA-B1474, Western blotting
All lanes: Anti Parathyroid Hormone Receptor 1 (ASA-B1474) at 0.5ug/ml
Lane 1: SKOV Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 66KD
All lanes: Anti Parathyroid Hormone Receptor 1 (ASA-B1474) at 0.5ug/ml
Lane 1: SKOV Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 66KD
Anti-Parathyroid Hormone Receptor 1 antibody, ASA-B1474, IHC(P)
IHC(P): Human Thyroid Cancer Tissue
IHC(P): Human Thyroid Cancer Tissue
