More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| pregnancy-associated plasma protein A, pappalysin 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PAPPA |
Protein | Pappalysin-1 |
Uniprot ID | Q13219 |
Function | Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. |
Tissue Specificity | High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the peptidase M43B family. |
Aliases | ASBABP2 antibody|Aspecific BCL2 ARE binding protein 2 antibody|Differentially placenta 1 expressed protein antibody|DIPLA1 antibody|IGF dependent IGFBP 4 protease antibody|IGF-dependent IGFBP-4 protease antibody|IGFBP 4ase antibody|IGFBP-4ase antibody|IGFBP4ase antibody|Insulin like growth factor dependent IGF binding protein 4 protease antibody|Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody|PAPA antibody|PAPP A antibody|PAPP-A antibody|PAPP1_HUMAN antibody|PAPPA antibody|PAPPA1 antibody|Pappalysin 1 precursor antibody|Pappalysin-1 antibody|Pregnancy Associated Plasma Protein A antibody|Pregnancy associated plasma protein A pappalysin 1 antibody|Pregnancy-associated plasma protein A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PAPP A antibody, ASA-B1473, Western blotting
All lanes: Anti PAPP A (ASA-B1473) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 181KD
Observed bind size: 181KD
All lanes: Anti PAPP A (ASA-B1473) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 181KD
Observed bind size: 181KD
Anti- PAPP A antibody, ASA-B1473, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
