More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cadherin 1, type 1, E-cadherin(epithelial); cadherin 2, type 1, N-cadherin(neuronal); cadherin 3, type 1, P-cadherin(placental) | Monoclonal | IgG | Mouse | Chicken, Human, Mouse, Rabbit, Rat, Snake | IHC-P, WB | Ascites |
Immunogen |
| Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue(24 amino acids) coupled to KLH. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDH1; CDH2; CDH3 |
Protein | Cadherin-1; Cadherin-2; Cadherin-3 |
Uniprot ID | Human(P12830), Mouse(P09803), Rat(Q9R0T4), Human(P19022), Mouse(P15116), Rat(Q9Z1Y3), Human(P22223), Mouse(P10287), Rat(F1LMI3) |
Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. |
Tissue Specificity | Non-neural epithelial tissues. |
Sub-cellular localization | Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. |
Sequence Similarities | Contains 5 cadherin domains. |
Aliases | UVO|CDHE|ECAD|LCAM|Arc-1|CD324|CDHN|NCAD|CD325|CDw325|CDHP|HJMD|PCAD |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2μg/ml | Human, mouse, rat, rabbit, chicken, snake. | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 2-4μg/ml | Human, mouse, rat, rabbit, chicken, snake. | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
