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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor protein p73 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human p73 recombinant protein (Position: M1-E198). Human p73 shares 96% amino acid (aa) sequence identity with mouse p73. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TP73 |
Protein | Tumor protein p73 |
Uniprot ID | O15350 |
Function | Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. |
Tissue Specificity | Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the p53 family. |
Aliases | p53 like transcription factor antibody|p53 Related Protein antibody|p53 Related Protein antibody|p53-like transcription factor antibody|p53-related protein antibody|p73 antibody|P73_HUMAN antibody|TP 73 antibody|TP73 antibody|TP73 antibody|Tumor Protein p73 antibody|Tumor Protein p73 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- p73 antibody, ASA-B1466, Western blotting
All lanes: Anti p73 (ASA-B1466) at 0.5ug/ml
WB: Recombinant Human p73 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti p73 (ASA-B1466) at 0.5ug/ml
WB: Recombinant Human p73 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- p73 antibody, ASA-B1466, Western blotting
All lanes: Anti p73 (ASA-B1466) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 73KD
Observed bind size: 73KD
All lanes: Anti p73 (ASA-B1466) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 73KD
Observed bind size: 73KD
Anti- p73 antibody, ASA-B1466, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue