More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor protein p63 | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TP63 |
Protein | Tumor protein 63 |
Uniprot ID | Q9H3D4 |
Function | Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. |
Tissue Specificity | Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the p53 family. |
Aliases | AIS antibody|Amplified in squamous cell carcinoma antibody|B(p51A) antibody|B(p51B) antibody|Chronic ulcerative stomatitis protein antibody|CUSP antibody|DN p63 alpha 1 antibody|DNp63 antibody|EEC3 antibody|Keratinocyte transcription factor antibody|Keratinocyte transcription factor KET antibody|KET antibody|LMS antibody|MGC115972 antibody|MGC192897 antibody|NBP antibody|OFC8 antibody|OTTHUMP00000209732 antibody|OTTHUMP00000209733 antibody|OTTHUMP00000209734 antibody|OTTHUMP00000209735 antibody|OTTHUMP00000209737 antibody|OTTHUMP00000209738 antibody|OTTHUMP00000209739 antibody|OTTHUMP00000209740 antibody|OTTHUMP00000209741 antibody|OTTHUMP00000209742 antibody|OTTHUMP00000209743 antibody|OTTHUMP00000209744 antibody|p40 antibody|p51 antibody|P51/P63 antibody|p53 like transcription factor antibody|p53 related protein antibody|p53-related protein p63 antibody|p53CP antibody|p63 antibody|P63_HUMAN antibody|P73 antibody|p73H antibody|p73L antibody|RHS antibody|SHFM4 antibody|TAp63alpha antibody|TP53CP antibody|TP53L antibody|TP63 antibody|TP73 antibody|TP73L antibody|Transformation related protein 63 antibody|Transformation-related protein 63 antibody|Trp53rp1 antibody|Trp63 antibody|Tumor protein 63 antibody|tumor protein 63 kDa with strong homology to p53 antibody|Tumor protein p53-competing protein antibody|Tumor protein p53-like antibody|tumor protein p63 antibody|Tumor protein p63 deltaN isoform delta antibody|Tumor protein p73 antibody|tumor protein p73-like antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-p63 antibody, ASA-B1465--1.jpg
All lanes: Anti P63 (ASA-B1465) at 0.5ug/ml
WB: Recombinant Human P63 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti P63 (ASA-B1465) at 0.5ug/ml
WB: Recombinant Human P63 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-p63 antibody, ASA-B1465--2.jpg
IHC(P): Human Oesophagus Squama Cancer Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue
