More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| purinergic receptor P2X, ligand gated ion channel, 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human P2X5 recombinant protein (Position: D333-T422). Human P2X5 shares 42% amino acid (aa) sequence identity with rat P2X5. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | P2RX5 |
Protein | P2X purinoceptor 5 |
Uniprot ID | Q93086 |
Function | Receptor for ATP that acts as a ligand-gated ion channel. |
Tissue Specificity | Expressed at high levels in brain and immune system. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the P2X receptor family. |
Aliases | ATP receptor antibody|ATP receptor subunit antibody|Ionotropic ATP receptor P2X5 antibody|LRH 1 antibody|LRH1 antibody|Lymphoid restricted histocompatibility antigen 1 antibody|MGC47755 antibody|P2rx5 antibody|P2RX5_HUMAN antibody|P2X purinoceptor 5 antibody|P2X5 antibody|P2X5R antibody|Purinergic receptor antibody|Purinergic receptor P2X ligand gated ion channel 5 antibody|Purinergic receptor P2X5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- P2X5 antibody, ASA-B1457, Western blotting
All lanes: Anti P2X5 (ASA-B1457) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti P2X5 (ASA-B1457) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
Anti- P2X5 antibody, ASA-B1457, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- P2X5 antibody, ASA-B1457, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue