More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| purinergic receptor P2X, ligand gated ion channel, 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | P2RX2 |
Protein | P2X purinoceptor 2 |
Uniprot ID | Q9UBL9 |
Function | Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the P2X receptor family. |
Aliases | ATP receptor antibody|MGC129601 antibody|P2RX 2 antibody|P2RX2 antibody|P2RX2_HUMAN antibody|P2X purinoceptor 2 antibody|P2X Receptor subunit 2 antibody|P2X2 antibody|P2X2a antibody|Purinergic receptor antibody|Purinergic receptor P2X ligand gated ion channel 2 antibody|Purinergic receptor P2X2 isoform A antibody|Purinergic receptor P2X2 isoform B antibody|Purinergic receptor P2X2 isoform C antibody|Purinergic receptor P2X2 isoform D antibody|Purinergic receptor P2X2 isoform H antibody|Purinergic receptor P2X2 isoform I antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- P2X2 antibody, ASA-B1456, Western blotting
All lanes: Anti P2X2 (ASA-B1456) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Lane 6: NEURO Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Lane 8: U87 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observ
All lanes: Anti P2X2 (ASA-B1456) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Lane 6: NEURO Whole Cell Lysate at 40ug
Lane 7: 22RV1 Whole Cell Lysate at 40ug
Lane 8: U87 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observ
Anti- P2X2 antibody, ASA-B1456, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
