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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cyclin-dependent kinase inhibitor 2A | Monoclonal | IgG | Mouse | Human | IHC-F, ICC, WB | Ascites |
Immunogen |
| Recombinant human p16 protein. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDKN2A |
Protein | Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 |
Uniprot ID | Human(P42771), Mouse(P51480), Rat(Q9R0Z3) |
Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. |
Aliases | ARF|MLM|P14|P16|P19|CMM2|INK4|MTS1|TP16|CDK4I|CDKN2|INK4A|MTS-1|P14ARF|P19ARF|P16INK4|P16INK4A|P16-INK4A|CDK4 INHIBITOR|cyclin-dependent kinase inhibitor 2A(melanoma, p16, inhibits CDK4)|Cyclin-dependent kinase 4 inhibitor A|Multiple tumor suppressor 1|Collaborator of ARF |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
