More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| retinoblastoma-like 1(p107) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human p107(1048-1068aa QENDDVLLKRLQDVVSERANH), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RBL1 |
Protein | Retinoblastoma-like protein 1 |
Uniprot ID | P28749 |
Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor. |
Tissue Specificity | |
Sub-cellular localization | Nucleus . |
Sequence Similarities | |
Aliases | 107 kDa retinoblastoma associated protein antibody|107 kDa retinoblastoma-associated protein antibody|Cellular protein 107 antibody|Cellular protein p107 antibody|CP 107 antibody|CP107 antibody|MGC40006 antibody|p107 antibody|PRB 1 antibody|PRB1 antibody|RB L1 antibody|RBL 1 antibody|RBL1 antibody|RBL1_HUMAN antibody|Retinoblastoma like 1 antibody|Retinoblastoma like protein 1 antibody|retinoblastoma-like 1(p107) antibody|Retinoblastoma-like protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-p107 antibody, ASA-B1447, Western blotting
Lane 1: 239T Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 1: 239T Cell Lysate
Lane 2: JURKAT Cell Lysate
Anti-p107 antibody, ASA-B1447, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-p107 antibody, ASA-B1447, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue