Osteocalcin  Antibody View larger

Osteocalcin Antibody

ASA-B1437

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

bone gamma-carboxyglutamate (gla) protein Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 86% amino acid (aa) sequences identity with mouse and rat Osteocalcin, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

BGLAP

Protein

Osteocalcin

Uniprot ID

P02818

Function

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Tissue Specificity

Sub-cellular localization

Secreted.

Sequence Similarities

Belongs to the osteocalcin/matrix Gla protein family.

Aliases

BGLAP antibody|BGP antibody|Bone gamma carboxyglutamate gla protein osteocalcin antibody|Bone gamma carboxyglutamate protein antibody|Bone Gla protein antibody|Gamma carboxyglutamic acid containing protein antibody|Gamma-carboxyglutamic acid-containing protein antibody|OC antibody|OSTCN_HUMAN antibody|Osteocalcin antibody|OTTHUMP00000016586 antibody|PMF1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Osteocalcin antibody, ASA-B1437--1.jpg
IHC(P): Human Osteosarcoma Tissue
Anti-Osteocalcin antibody, ASA-B1437--2.jpg
All lanes: Anti-Osteocalcin(ASA-B1437) at 0.5ug/ml
WB: Recombinant Human Osteocalcin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD

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