More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hypocretin(orexin) receptor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Orexin Receptor 1(327-342aa FRQASDREAVYACFTF), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HCRTR1 |
Protein | Orexin receptor type 1(Ox-1-R/Ox1-R/Ox1R) |
Uniprot ID | O43613 |
Function | Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | HCRTR 1 antibody|HCRTR1 antibody|Hypocretin receptor type 1 antibody|Orexin receptor type 1 antibody|Ox-1-R antibody|Ox1-R antibody|Ox1r antibody|OX1R_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Orexin Receptor 1 antibody, ASA-B1435, Western blotting
WB: Rat Brain Tissue Lysate
WB: Rat Brain Tissue Lysate
Anti-Orexin Receptor 1 antibody, ASA-B1435, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
