Orexin Receptor 1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
hypocretin(orexin) receptor 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Orexin Receptor 1(327-342aa FRQASDREAVYACFTF), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HCRTR1
Protein	Orexin receptor type 1(Ox-1-R/Ox1-R/Ox1R)
Uniprot ID	O43613
Function	Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade (By similarity).
Tissue Specificity
Sub-cellular localization	Cell membrane; Multi-pass membrane protein.
Sequence Similarities
Aliases	HCRTR 1 antibody|HCRTR1 antibody|Hypocretin receptor type 1 antibody|Orexin receptor type 1 antibody|Ox-1-R antibody|Ox1-R antibody|Ox1r antibody|OX1R_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information