More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| POU class 2 homeobox 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | POU2F1 |
Protein | POU domain, class 2, transcription factor 1 |
Uniprot ID | P14859 |
Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. |
Tissue Specificity | Ubiquitous. Isoform 2 is lymphocyte-specific. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the POU transcription factor family. Class- 2 subfamily. |
Aliases | FLJ42836 antibody|NF A1 antibody|NF-A1 antibody|Oct 1 antibody|Oct 1B antibody|Oct-1 antibody|OCT1 antibody|Octamer binding protein 1 antibody|Octamer binding transcription factor 1 antibody|Octamer-binding protein 1 antibody|Octamer-binding transcription factor 1 antibody|OTF 1 antibody|OTF-1 antibody|OTF1 antibody|OTTHUMP00000032348 antibody|OTTHUMP00000032350 antibody|OTTHUMP00000032351 antibody|PO21 antibody|PO2F1 antibody|PO2F1_HUMAN antibody|POU class 2 homeobox 1 antibody|POU domain class 2 transcription factor 1 antibody|POU domain, class 2, transcription factor 1 antibody|POU2F1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Oct-1 antibody, ASA-B1429, Western blotting
All lanes: Anti Oct-1 (ASA-B1429) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
All lanes: Anti Oct-1 (ASA-B1429) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
Anti- Oct-1 antibody, ASA-B1429, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
