More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| occludin | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | OCLN |
Protein | Occludin |
Uniprot ID | Q16625 |
Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. |
Tissue Specificity | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. Cell junction, tight junction. |
Sequence Similarities | Belongs to the ELL/occludin family. |
Aliases | BLCPMG antibody|FLJ08163 antibody|FLJ18079 antibody|FLJ77961 antibody|FLJ94056 antibody|MGC34277 antibody|Occludin antibody|OCLN antibody|OCLN_HUMAN antibody|Tight junction protein occludin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Occludin antibody, ASA-B1428, Western blotting
All lanes: Anti Occludin (ASA-B1428) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
All lanes: Anti Occludin (ASA-B1428) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
Anti- Occludin antibody, ASA-B1428,IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
