More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nucleophosmin (nucleolar phosphoprotein B23, numatrin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Nucleophosmin recombinant protein (Position: M1-L294). Human Nucleophosmin shares 95% amino acid (aa) sequence identity with both mouse and rat Nucleophosmin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NPM1 |
Protein | Nucleophosmin |
Uniprot ID | P06748 |
Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. |
Tissue Specificity | |
Sub-cellular localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis. |
Sequence Similarities | Belongs to the nucleoplasmin family. |
Aliases | B23 antibody|MGC104254 antibody|NMP1 antibody|NO38 antibody|NPM 1 antibody|NPM antibody|NPM_HUMAN antibody|NPM1 antibody|Nucleolar Phosphoprotein B23 antibody|Nucleolar protein NO38 antibody|Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody|Nucleophosmin antibody|nucleophosmin nucleoplasmin family member 1 antibody|Nucleophosmin/B23.2 antibody|Nucleophosmin/nucleoplasmin family member 1 antibody|Nucleoplasmin Family Member 1 antibody|Numatrin antibody|OTTHUMP00000161024 antibody|OTTHUMP00000161025 antibody|OTTHUMP00000223397 antibody|OTTHUMP00000223398 antibody|TRK fused gene antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Nucleophosmin antibody, ASA-B1423, Western blotting
All lanes: Anti Nucleophosmin (ASA-B1423) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: HUT Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 38KD
All lanes: Anti Nucleophosmin (ASA-B1423) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: HUT Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 38KD
Anti- Nucleophosmin antibody, ASA-B1423,IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Nucleophosmin antibody, ASA-B1423,IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue