More info
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear factor(erythroid-derived 2)-like 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Nrf2(580-596aa EYSLQQTRDGNVFLVPK), identical to the related mouse and rat sequences. | ||||||
Purification method Affinity purification
Isotype IgG
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Additional Information Regarding the Antigen
Additional Information Regarding the Antigen
Gene |
NFE2L2 |
Protein |
Nuclear factor erythroid 2-related factor 2 |
Uniprot ID |
Q16236 |
Function |
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. |
Tissue Specificity |
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. |
Sub-cellular localization |
Cytoplasm, cytosol. Nucleus. Note: Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. |
Sequence Similarities |
Belongs to the bZIP family. CNC subfamily. |
Aliases |
erythroid derived 2 antibody|HEBP1 antibody|like 2 antibody|NF E2 related factor 2 antibody|NF-E2-related factor 2 antibody|NF2L2_HUMAN antibody|NFE2 related factor 2 antibody|NFE2-related factor 2 antibody|NFE2L2 antibody|Nrf 2 antibody|NRF2 antibody|Nuclear factor antibody|nuclear factor erythroid 2 like 2 antibody|Nuclear factor erythroid 2 related factor 2 antibody|Nuclear factor erythroid 2-related factor 2 antibody|Nuclear factor erythroid derived 2 like 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Nrf2 antibody, ASA-B1413, Western blotting
All lanes: Anti Nrf2 (ASA-B1413) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: MM231Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
All lanes: Anti Nrf2 (ASA-B1413) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: MM231Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
Anti-Nrf2 antibody, ASA-B1413, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-Nrf2 antibody, ASA-B1413, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue