NRF1 Antibody View larger

NRF1 Antibody

ASA-B1412

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

nuclear respiratory factor 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

NRF1

Protein

Nuclear respiratory factor 1

Uniprot ID

Q16656

Function

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Tissue Specificity

Ubiquitously expressed with strongest expression in skeletal muscle.

Sub-cellular localization

Nucleus.

Sequence Similarities

Belongs to the NRF1/Ewg family.

Aliases

alpha pal antibody|alpha palindromic binding protein antibody|Alpha palindromic-binding protein antibody|Alpha-pal antibody|locus control region factor 1 antibody|NFE2 related factor 1 antibody|NRF-1 antibody|Nrf1 antibody|NRF1_HUMAN antibody|nuclear factor(erythroid derived 2)-like 1 antibody|nuclear respiratory factor 1 antibody|transcription factor 11 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NRF1 antibody, ASA-B1412, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: A549 Cell Lysate

Anti-NRF1 antibody, ASA-B1412, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-NRF1 antibody, ASA-B1412, ICC
ICC: A549 Cell

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