More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human NR3C1 recombinant protein (Position: M1-D373). Human NR3C1 shares 85% and 82% amino acid (aa) sequences identity with mouse and rat NR3C1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NR3C1 |
Protein | Glucocorticoid receptor |
Uniprot ID | P04150 |
Function | Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. |
Tissue Specificity | Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR3 subfamily. |
Aliases | GCCR antibody|GCR antibody|GCR_HUMAN antibody|GCRST antibody|glucocorticoid nuclear receptor variant 1 antibody|Glucocorticoid receptor antibody|Glucocorticoid receptor lymphocyte antibody|GR antibody|GRL antibody|Grl1 antibody|NR3C1 antibody|Nuclear receptor subfamily 3 group C member 1 antibody|nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NR3C1 antibody, ASA-B1409, Western blotting
All lanes: Anti NR3C1 (ASA-B1409) at 0.5ug/ml
WB: Recombinant Human NR3C1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti NR3C1 (ASA-B1409) at 0.5ug/ml
WB: Recombinant Human NR3C1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- NR3C1 antibody, ASA-B1409, Western blotting
All lanes: Anti NR3C1 (ASA-B1409) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
All lanes: Anti NR3C1 (ASA-B1409) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
Anti- NR3C1 antibody, ASA-B1409,IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue