NPHS2 Antibody View larger

NPHS2 Antibody

ASA-B1405

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

nephrosis 2, idiopathic, steroid-resistant(podocin) Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NPHS2(368-383aa KPVEPLNPKKKDSPML), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

NPHS2

Protein

Podocin

Uniprot ID

Q9NP85

Function

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Tissue Specificity

Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Sub-cellular localization

Isoform 1: Cell membrane ; Peripheral membrane protein .

Sequence Similarities

Belongs to the band 7/mec-2 family.

Aliases

Nephrosis 2, idiopathic, steroid resistant(podocin) antibody|nephrosis 2, idiopathic, steroid resistant antibody|NPHS2 antibody|NPHS2 gene antibody|PDCN antibody|PODO_HUMAN antibody|Podocin antibody|SRN1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Rat Human, MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat Human, MouseAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Rat Human, MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NPHS2 antibody, ASA-B1405, Western blotting
WB: Rat Kidney Tissue Lysate
Anti-NPHS2 antibody, ASA-B1405, IHC(F)
IHC(F): Rat Kidney Tissue

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