More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| reticulon 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Nogo A(1170-1192aa NKNVKDAMAKIQAKIPGLKRKAE), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RTN4 |
Protein | Reticulon-4 |
Uniprot ID | Q9NQC3 |
Function | Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. |
Tissue Specificity | Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific. |
Sub-cellular localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. |
Sequence Similarities | Contains 1 reticulon domain. |
Aliases | ASY antibody|Foocen antibody|Human NogoA antibody|KIAA0886 antibody|My043 protein antibody|Nbla00271 antibody|Nbla10545 antibody|Neurite growth inhibitor 220 antibody|Neurite Outgrowth Inhibitor antibody|Neuroendocrine specific protein antibody|Neuroendocrine specific protein C homolog antibody|Neuroendocrine-specific protein antibody|Neuroendocrine-specific protein C homolog antibody|NI220/250 antibody|Nogo A antibody|NOGO antibody|Nogo protein antibody|NOGO-A antibody|Nogo-B antibody|Nogo-C antibody|NogoA antibody|NSP antibody|NSP CL antibody|NSP-CL antibody|Reticulon 4 antibody|Reticulon 5 antibody|Reticulon-4 antibody|Reticulon-5 antibody|Reticulon4 antibody|Reticulon5 antibody|RTN 4 antibody|RTN 4A antibody|RTN X antibody|RTN xL antibody|RTN-x antibody|RTN4 A antibody|RTN4 antibody|RTN4 B1 antibody|RTN4 B2 antibody|RTN4 C antibody|RTN4 protein antibody|RTN4-A antibody|RTN4-B1 antibody|RTN4-B2 antibody|RTN4-C antibody|RTN4_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Nogo A antibody, ASA-B1396, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Anti-Nogo A antibody, ASA-B1396, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
