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nNOS(neuronal) Antibody View larger

nNOS(neuronal) Antibody

ASA-B1395

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
nitric oxide synthase 1(neuronal) Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal)(1418-1434aa IAFIEESKKDTDEVFSS), identical to the related mouse and rat sequences.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 NOS1

Protein

 Nitric oxide synthase, brain

Uniprot ID

 P29475

Function

 Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Tissue Specificity

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Sub-cellular localization

 Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine . Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).

Sequence Similarities

 Belongs to the NOS family.

Aliases

 2310005C01Rik antibody|bNOS antibody|Constitutive NOS antibody|EC 1.14.13.39 antibody|IHPS 1 antibody|IHPS1 antibody|N NOS antibody|N-NOS antibody|NC NOS antibody|NC-NOS antibody|neuronal Nitric Oxide Synthase antibody|Neuronal NOS antibody|Nitric oxide synthase, neuronal, included antibody|Nitric oxide synthase 1(neuronal) antibody|Nitric oxide synthase 1 antibody|Nitric oxide synthase brain antibody|Nitric oxide synthase, brain antibody|Nitric oxide synthase, penile neuronal, included antibody|nNOS antibody|NO antibody|NOS 1 antibody|NOS antibody|NOS type I antibody|NOS-I antibody|NOS1 antibody|NOS1_HUMAN antibody|Peptidyl-cysteine S-nitrosylase NOS1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Rat Human, MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-nNOS(neuronal) antibody, ASA-B1395, Western blotting
All lanes: Anti nNOS(neuronal)(ASA-B1395) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 160KD
Observed bind size: 160KD
Anti-nNOS(neuronal) antibody, ASA-B1395, IHC(P)
IHC(P): Rat Brain Tissue

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