More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| NME/NM23 nucleoside diphosphate kinase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NME2(137-152aa EELVDYKSCAHDWVYE), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NME2 |
Protein | Nucleoside diphosphate kinase B(NDK B) |
Uniprot ID | P22392 |
Function | Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Note: Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates. |
Sequence Similarities | |
Aliases | C myc purine binding transcription factor PUF antibody|C myc transcription factor antibody|C-myc purine-binding transcription factor PUF antibody|Histidine protein kinase NDKB antibody|MGC111212 antibody|MGC2212 antibody|NDK B antibody|NDKB antibody|NDKB_HUMAN antibody|NDP kinase B antibody|NDPK B antibody|NDPKB antibody|NM23 H2 antibody|nm23-H2 antibody|NM23B antibody|nme2 antibody|Non metastatic cells 2, protein(NM23B) expressed in antibody|Nucleoside diphosphate kinase B antibody|Nucleotide diphosphate kinase B antibody|PUF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NME2 antibody, ASA-B1393, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: PANC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: U87 Cell Lysate
Lane 9: COLO320 Cell Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: PANC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: U87 Cell Lysate
Lane 9: COLO320 Cell Lysate
Anti-NME2 antibody, ASA-B1393, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-NME2 antibody, ASA-B1393, IHC(P)
IHC(P): Rat Cerebellum Tissue
IHC(P): Rat Cerebellum Tissue