More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human NFkB p105/P50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NFKB1 |
Protein | Nuclear factor NF-kappa-B p105 subunit |
Uniprot ID | P19838 |
Function | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
Sequence Similarities | Contains 7 ANK repeats. |
Aliases | DKFZp686C01211 antibody|DNA binding factor KBF1 antibody|DNA binding factor KBF1 EBP1 antibody|DNA-binding factor KBF1 antibody|EBP 1 antibody|EBP-1 antibody|EBP1 antibody|KBF1 antibody|MGC54151 antibody|NF kappa B antibody|NF kappaB antibody|NF kappabeta antibody|NF kB1 antibody|NFkappaB antibody|NFKB 1 antibody|NFKB p105 antibody|NFKB p50 antibody|NFKB1 antibody|NFKB1_HUMAN antibody|Nuclear factor kappa B DNA binding subunit antibody|Nuclear factor kappa-B, subunit 1 antibody|Nuclear factor NF kappa B p105 subunit antibody|Nuclear factor NF kappa B p50 subunit antibody|Nuclear factor NF-kappa-B p50 subunit antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody|Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody|p105 antibody|p50 antibody|p84/NF-kappa-B1 p98 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NFkB p105/P50 antibody, ASA-B1377--1.jpg
All lanes: Anti NFKBP105 (ASA-B1377) at 0.5ug/ml
WB: Recombinant Human NFKBP105 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti NFKBP105 (ASA-B1377) at 0.5ug/ml
WB: Recombinant Human NFKBP105 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-NFkB p105/P50 antibody, ASA-B1377--2.jpg
All lanes: Anti NFKBP105 (ASA-B1377) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti NFKBP105 (ASA-B1377) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-NFkB p105/P50 antibody, ASA-B1377--3.jpg
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue