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NF200 Antibody (monoclonal) View larger

NF200 Antibody (monoclonal)

ASA-B1368

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
neurofilament, heavy polypeptide Monoclonal IgG Mouse Human, Mouse, Rat IHC-P, IHC-F, WB Ascites

Immunogen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 NEFH

Protein

 Neurofilament heavy polypeptide

Uniprot ID

 Human(P12036), Mouse(P19246), Rat(P16884)

Function

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Tissue Specificity

Sub-cellular localization

Sequence Similarities

 Belongs to the intermediate filament family.

Aliases

 NFH|KIAA0845|200 kDa neurofilament protein|Neurofilament triplet H protein

Application Details

Application Concentration* Species Validated Using**
Western blot0.5mlHuman, mouse, ratAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)1-2μg/mlHuman, mouse, ratAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)1-2μg/mlHuman, mouse, ratAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NF200 antibody (monoclonal), ASA-B1368, IHC(P)
IHC(P): Rat Brain Tissue

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