More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| neurotrophin 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Neurotrophin 3(174-189aa TVLGEIKTGNSPVKQY), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NTF3 |
Protein | Neurotrophin-3(NT-3) |
Uniprot ID | P20783 |
Function | Seems to promote the survival of visceral and proprioceptive sensory neurons. |
Tissue Specificity | Brain and peripheral tissues. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | HDNF antibody|MGC129711 antibody|Nerve growth factor 2 antibody|Neurotrophic factor antibody|Neurotrophin 3 precursor antibody|Neurotrophin-3 antibody|Neurotrophin3 antibody|NGF 2 antibody|NGF-2 antibody|NGF2 antibody|NT 3 antibody|NT-3 antibody|NT3 antibody|NTF 3 antibody|NTF3 antibody|NTF3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Neurotrophin 3 antibody, ASA-B1363, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HELA Cell Lysate
Anti-Neurotrophin 3 antibody, ASA-B1363, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
