More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| neuropilin 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NRP1 |
Protein | Neuropilin-1 |
Uniprot ID | O14786 |
Function | The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. |
Tissue Specificity | The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the neuropilin family. |
Aliases | A5 protein antibody|BDCA4 antibody|BLOOD DENDRITIC CELL ANTIGEN 4 antibody|CD 304 antibody|CD304 antibody|DKFZp686A03134 antibody|DKFZp781F1414 antibody|Neuropilin-1 antibody|Neuropilin1 antibody|Neuropilin1 transmembrane receptor antibody|NP1 antibody|NPN1 antibody|NRP 1 antibody|NRP antibody|NRP1 antibody|NRP1_HUMAN antibody|Vascular endothelial cell growth factor 165 receptor antibody|VEGF165R antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Neuropillin-1 antibody, ASA-B1362, Western blotting
All lanes: Anti Neuropillin-1 (ASA-B1362) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 103KD
Observed bind size: 103KD
All lanes: Anti Neuropillin-1 (ASA-B1362) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 103KD
Observed bind size: 103KD
Anti- Neuropillin-1 antibody, ASA-B1362, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
