More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nephrosis 1, congenital, Finnish type (nephrin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Nephrin recombinant protein (Position: Q23-P383). Human Nephrin shares 79% and 80% amino acid (aa) sequences identity with mouse and rat Nephrin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NPHS1 |
Protein | Nephrin |
Uniprot ID | O60500 |
Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). |
Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli. |
Sub-cellular localization | Cell membrane ; Single-pass type I membrane protein . Note: Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. |
Sequence Similarities | Belongs to the immunoglobulin superfamily. |
Aliases | CNF antibody|Nephrin antibody|Nephrosis 1 congenital Finnish type antibody|Nephrosis 1, congenital, Finnish type (nephrin) antibody|NPHN antibody|NPHN_HUMAN antibody|NPHS 1 antibody|NPHS1 antibody|Renal glomerulus specific cell adhesion receptor antibody|Renal glomerulus-specific cell adhesion receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Nephrin antibody, ASA-B1357--1.jpg
All lanes: Anti Nephrin (ASA-B1357) at 0.5ug/ml
WB: Recombinant Human Nephrin Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti Nephrin (ASA-B1357) at 0.5ug/ml
WB: Recombinant Human Nephrin Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-Nephrin antibody, ASA-B1357--2.jpg
All lanes: Anti Nephrin (ASA-B1357) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 134KD
Observed bind size: 134KD
All lanes: Anti Nephrin (ASA-B1357) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 134KD
Observed bind size: 134KD
Anti-Nephrin antibody, ASA-B1357--3.jpg
IHC(F): Rat Kidney Tissue
IHC(F): Rat Kidney Tissue