More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| NADH dehydrogenase(ubiquinone) 1 alpha subcomplex, 1, 7.5kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NDUFA1 |
Protein | NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 |
Uniprot ID | O15239 |
Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
Tissue Specificity | Primarily expressed in heart and skeletal muscle. |
Sub-cellular localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. |
Sequence Similarities | |
Aliases | CI MWFE antibody|CI-MWFE antibody|Complex I MWFE antibody|complex I MWFE subunit antibody|Complex I-MWFE antibody|MWFE antibody|MWFE, B. taurus, homolog of antibody|NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 7.5kDa antibody|NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 antibody|NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 antibody|NADH oxidoreductase subunit MWFE antibody|NADH ubiquinone oxidoreductase(complex 1) antibody|NADH ubiquinone oxidoreductase MWFE subunit antibody|NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 antibody|NADH-ubiquinone oxidoreductase MWFE subunit antibody|NDUA1_HUMAN antibody|NDUFA 1 antibody|NDUFA1 antibody|Type I dehydrogenase antibody|ZNF 183 antibody|ZNF183 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NDUFA1 antibody, ASA-B1355, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: HT1080 Cell Lysate
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: HT1080 Cell Lysate
Anti-NDUFA1 antibody, ASA-B1355, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
Anti-NDUFA1 antibody, ASA-B1355, IHC(F)
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue