More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| NADPH oxidase 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NOX4 |
Protein | NADPH oxidase 4 |
Uniprot ID | Q9JHI8 |
Function | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. |
Tissue Specificity | EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. |
Sub-cellular localization | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . Cell membrane . Note: May localize to plasma membrane and focal adhesions. |
Sequence Similarities | Contains 1 FAD-binding FR-type domain. |
Aliases | Kidney oxidase-1 antibody|Kidney superoxide-producing NADPH oxidase antibody|KOX 1 antibody|KOX antibody|Kox-1 antibody|NADPH antibody|NADPH oxidase 4 antibody|Nox4 antibody|NOX4_HUMAN antibody|Renal NAD(P)H-oxidase antibody|RENOX antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NADPH oxidase 4 antibody, ASA-B1347, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: SMMC Cell Lysate
Anti-NADPH oxidase 4 antibody, ASA-B1347, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
