N Cadherin Antibody View larger

N Cadherin Antibody

ASA-B1346

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

cadherin 2, type 1, N-cadherin(neuronal) Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin(701-714aa CQCDSNGDCTDVDR), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CDH2

Protein

Cadherin-2

Uniprot ID

P19022

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Tissue Specificity

Sub-cellular localization

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Aliases

CADH2_HUMAN antibody|Cadherin 2 antibody|Cadherin 2 N cadherin neuronal antibody|Cadherin 2 type 1 antibody|Cadherin 2 type 1 N cadherin neuronal antibody|cadherin 2 type 1 N-cadherin neuronal antibody|Cadherin 2, type 1, N-cadherin(neuronal) antibody|Cadherin-2 antibody|Cadherin2 antibody|Calcium dependent adhesion protein neuronal antibody|CD325 antibody|CD325 antigen antibody|CDH2 antibody|CDHN antibody|CDw325 antibody|CDw325 antigen antibody|N cadherin 1 antibody|N-cadherin antibody|NCAD antibody|Neural Cadherin antibody|OTTHUMP00000066304 antibody|OTTHUMP00000067378 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-N Cadherin antibody, ASA-B1346, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-N Cadherin antibody, ASA-B1346, IHC(P)
IHC(P): Human Ovary Cancer Tissue

© 2024 Novateinbio.com