More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
Myeloperoxidase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human Myeloperoxidase(714-728aa KNNIFMSNSYPRDFV), different from the related mouse and rat sequences by one amino acid. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MPO |
Protein | Myeloperoxidase(MPO) |
Uniprot ID | P05164 |
Function | Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. |
Tissue Specificity | |
Sub-cellular localization | Lysosome. |
Sequence Similarities | Belongs to the peroxidase family. XPO subfamily. |
Aliases | 84 kDa myeloperoxidase antibody|89 kDa myeloperoxidase antibody|EC 1.11.1.7 antibody|EC1.11.2.2 antibody|MPO antibody|Myeloperoxidase heavy chain antibody|Myeloperoxidase light chain antibody|PERM_HUMAN antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Myeloperoxidase antibody, ASA-B1336, Western blotting
WB: Rat Brain Tissue Lysate
Anti-Myeloperoxidase antibody, ASA-B1336, IHC(P)
IHC(P): Human Liver Cancer Tissue