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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interferon regulatory factor 4 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IRF4 |
Protein | Interferon regulatory factor 4 |
Uniprot ID | Q15306 |
Function | Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). |
Tissue Specificity | Lymphoid cells. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the IRF family. |
Aliases | Interferon regulatory factor 4 antibody|IRF 4 antibody|IRF-4 antibody|Irf4 antibody|IRF4_HUMAN antibody|LSIRF antibody|Lymphocyte specific interferon regulatory factor antibody|Lymphocyte specific IRF antibody|Lymphocyte-specific interferon regulatory factor antibody|Multiple myeloma oncogene 1 antibody|MUM 1 antibody|MUM1 antibody|NF EM5 antibody|NF-EM5 antibody|NFEM5 antibody|PU.1 interaction partner antibody|Sfpi1/PU.1 interaction partner antibody|Transcriptional activator PIP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MUM1 antibody, ASA-B1330, Western blotting
All lanes: Anti MUM1 (ASA-B1330) at 0.5ug/ml
WB: Recombinant Human MUM1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti MUM1 (ASA-B1330) at 0.5ug/ml
WB: Recombinant Human MUM1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- MUM1 antibody, ASA-B1330, Western blotting
All lanes: Anti MUM1 (ASA-B1330) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 40KD
All lanes: Anti MUM1 (ASA-B1330) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 40KD
Anti- MUM1 antibody, ASA-B1330, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue