More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mucin 5AC, oligomeric mucus/gel-forming | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC(552-567aa TMQLFMQLAPKLRGQT). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MUC5AC |
Protein | Mucin-5AC |
Uniprot ID | P98088 |
Function | Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. |
Tissue Specificity | Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 CTCK (C-terminal cystine knot-like) domain. |
Aliases | Apomucin antibody|Gastric mucin antibody|Major Airway Glycoprotein antibody|MUC 5 antibody|MUC5 antibody|MUC5AC antibody|Mucin 5 subtype AC tracheobronchial antibody|Mucin 5 Subtypes A And C antibody|Mucin 5 subtypes A and C tracheobronchial/gastric antibody|Mucin 5AC oligomeric mucus/gel forming antibody|TBM antibody|Tracheobronchial Mucin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Mucin 5AC antibody, ASA-B1327, All Western blotting
All lanes: Anti-MUC5AC(ASA-B1327) at0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 527KD
Observed bind size: 300KD
All lanes: Anti-MUC5AC(ASA-B1327) at0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 527KD
Observed bind size: 300KD
