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MUC1 Antibody

ASA-B1323

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
mucin 1, cell surface associated Polyclonal IgG Rabbit Mouse, Rat IHC-P, IHC-F, WB Immunogen affinity purified.

Immunogen

E.coli-derived mouse MUC1 recombinant protein (Position: S474-L630). Mouse MUC1 shares 87% and amino acid (aa) sequence identity with human MUC1.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 MUC1, MUC1

Protein

 Mucin-1

Uniprot ID

 Q02496

Function

 The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Tissue Specificity

Expressed in a variety of epithelial tissues.

Sub-cellular localization

 Apical cell membrane; Single-pass type I membrane protein. Note: Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions (By similarity).

Sequence Similarities

 Contains 1 SEA domain.

Aliases

 ADMCKD antibody|ADMCKD1 antibody|Breast carcinoma associated antigen DF3 antibody|Breast carcinoma-associated antigen DF3 antibody|CA 15-3 antibody|CA15 3 antibody|CA15 3 antigen antibody|CA15.3 antibody|Cancer antigen 15-3 antibody|Carcinoma associated mucin antibody|Carcinoma-associated mucin antibody|CD 227 antibody|CD227 antibody|DF3 antigen antibody|EMA antibody|Episialin antibody|H23 antigen antibody|H23AG antibody|KL 6 antibody|KL-6 antibody|KL6 antibody|Krebs von den Lungen-6 antibody|MAM 6 antibody|MAM6 antibody|MCD antibody|MCKD antibody|MCKD1 antibody|Medullary cystic kidney disease 1 (autosomal dominant) antibody|MUC 1 antibody|MUC-1 antibody|MUC-1/SEC antibody|MUC-1/X antibody|MUC1 antibody|MUC1-alpha antibody|MUC1-beta antibody|MUC1-CT antibody|MUC1-NT antibody|MUC1/ZD antibody|MUC1_HUMAN antibody|Mucin 1 antibody|Mucin 1 transmembrane antibody|Mucin 1, cell surface associated antibody|Mucin-1 subunit beta antibody|Peanut reactive urinary mucin antibody|Peanut-reactive urinary mucin antibody|PEM antibody|PEMT antibody|Polymorphic epithelial mucin antibody|PUM antibody|Tumor associated epithelial membrane antigen antibody|Tumor associated epithelial mucin antibody|Tumor associated mucin antibody|Tumor-associated epithelial membrane antigen antibody|Tumor-associated mucin antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MUC1 antibody, ASA-B1323--1.jpg
All lanes: Anti MUC1 (ASA-B1323) at 0.5ug/ml
WB: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
Anti-MUC1 antibody, ASA-B1323--2.jpg
IHC(P): Rat Kidney Tissue
Anti-MUC1 antibody, ASA-B1323--3.jpg
IHC(P): Mouse Lung Tissue

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