More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transcription factor A, mitochondrial | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TFAM |
Protein | Transcription factor A, mitochondrial |
Uniprot ID | Q00059 |
Function | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. |
Sequence Similarities | |
Aliases | anscription factor 6-like 1 antibody|Mitochondrial transcription factor 1 antibody| mitochondrial transcription factor A antibody|MtTF1 antibody|mtTFA antibody|TCF 6 antibody| TCF-6 antibody|TCF6 antibody|TCF6L1 antibody|TCF6L2 antibody| TCF6L3 antibody|TFAM antibody| TFAM_HUMAN antibody|Transcription factor 6 antibody|Transcription factor 6 like 2 (mitochondrial transcription factor) antibody| Transcription factor 6 like 2 antibody| Transcription factor 6-like 2 antibody| transcription factor 6-like 3 antibody|Transcription factor A, mitochondrial antibody| Transcription factor A, mitochondrial precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- mtTFA antibody, ASA-B1321, Western blotting
All lanes: Anti mtTFA (ASA-B1321) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti mtTFA (ASA-B1321) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
Anti- mtTFA antibody, ASA-B1321,IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
