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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitochondrially encoded cytochrome c oxidase I | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse MTCO1(501-514aa PYHTFEEPTYVKVK), different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MT-CO1 |
Protein | Cytochrome c oxidase subunit 1 |
Uniprot ID | P00397 |
Function | Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion inner membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | COI antibody|COX I antibody|COX1_HUMAN antibody|COXI antibody|Cytochrome c oxidase polypeptide I antibody|Cytochrome c oxidase subunit 1 antibody|Cytochrome C Oxidase subunit I antibody|Mitochondrially encoded cytochrome c oxidase I antibody|MT CO1 antibody|MT-CO1 antibody|MTCO 1 antibody|MTCO1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Mouse Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MTCO1 antibody, ASA-B1318, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Anti-MTCO1 antibody, ASA-B1318, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-MTCO1 antibody, ASA-B1318, IHC(P)
IHC(P): Mouse Liver Tissue
IHC(P): Mouse Liver Tissue