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MSK1 Antibody

ASA-B1315

$365.00

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Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
ribosomal protein S6 kinase, 90kDa, polypeptide 5 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

E.coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 RPS6KA5

Protein

 Ribosomal protein S6 kinase alpha-5

Uniprot ID

 O75582

Function

 Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto- oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro- inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti- inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.

Tissue Specificity

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Sub-cellular localization

 Nucleus. Cytoplasm. Note: Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.

Sequence Similarities

 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Aliases

 90 kDa ribosomal protein S6 kinase 5 antibody|KS6A5_HUMAN antibody|MGC1911 antibody|Mitogen and stress activated protein kinase 1 antibody|MSPK1 antibody|Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody|Nuclear mitogen- and stress-activated protein kinase 1 antibody|Ribosomal protein S6 kinase 90kD polypeptide 5 antibody|Ribosomal protein S6 kinase 90kDa antibody|Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody|Ribosomal Protein S6 Kinase Alpha 5 antibody|Ribosomal protein S6 kinase alpha-5 antibody|RLPK antibody|RPS6KA5 antibody|RSK Like Protein Kinase antibody|RSK-like protein kinase antibody|RSKL antibody|S6K alpha 5 antibody|S6K-alpha-5 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MSK1 antibody, ASA-B1315, Western blotting
All lanes: Anti MSK1 (ASA-B1315) at 0.5ug/ml
WB: Recombinant Human MSK1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- MSK1 antibody, ASA-B1315, Western blotting
All lanes: Anti MSK1 (ASA-B1315) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
Anti- MSK1 antibody, ASA-B1315, IHC(P)
IHC(P): Mouse Cardiac Muscle Tissue

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