More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 16, member 1(monocarboxylic acid transporter 1) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Monocarboxylic acid transporter 1(236-254aa RHPKQEKRSVFQTINQFLD). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC16A1 |
Protein | Monocarboxylate transporter 1 |
Uniprot ID | P53985 |
Function | Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis. |
Tissue Specificity | Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. |
Aliases | FLJ36745 antibody|HHF7 antibody|MCT 1 antibody|MCT antibody|MGC44475 antibody|Monocarboxylate transporter 1 antibody|monocarboxylate transporter antibody|Monocarboxylate transporter isoform 1 antibody|Monocarboxylic acid transporter 1 antibody|SLC16A1 antibody|SLC16A1 protein antibody|Solute carrier family 16(monocarboxylic acid transporters) member 1 antibody|Solute carrier family 16 member 1(monocarboxylic acid transporter 1) antibody|Solute carrier family 16 member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Monocarboxylic acid transporter 1 antibody, ASA-B1302, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: SKOV Cell Lysate
Lane 3: A549 Cell Lysate
Lane 1: COLO320 Cell Lysate
Lane 2: SKOV Cell Lysate
Lane 3: A549 Cell Lysate
Anti-Monocarboxylic acid transporter 1 antibody, ASA-B1302, IHC(P)
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue
