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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| MNAT CDK-activating kinase assembly factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MNAT1 recombinant protein (Position: L92-A278). Human MNAT1 shares 93% amino acid (aa) sequence identity with mouse MNAT1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MNAT1 |
Protein | CDK-activating kinase assembly factor MAT1 |
Uniprot ID | P51948 |
Function | Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. |
Tissue Specificity | Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 RING-type zinc finger. |
Aliases | MNAT 1 antibody|MNAT 1 antibody|CAP35 antibody|CDK activating kinase assembly factor MAT1 antibody|CDK-activating kinase assembly factor MAT1 antibody|CDK7/cyclin H assembly factor antibody|CDK7/cyclin-H assembly factor antibody|Cyclin G1 interacting protein antibody| Cyclin-G1-interacting protein antibody|MAT1 antibody|MAT1_HUMAN antibody|Menage a trois 1 (CAK assembly factor) antibody| Menage a trois antibody|Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody|MNAT CDK activating kinase assembly factor 1 antibody|Mnat1 antibody|p35 antibody|p36 antibody|RING finger protein 66 antibody|RING finger protein MAT1 antibody| RNF66 antibody|TFB3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MNAT1 antibody, ASA-B1299, Western blotting
All lanes: Anti MNAT1 (ASA-B1299) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
All lanes: Anti MNAT1 (ASA-B1299) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
Anti- MNAT1 antibody, ASA-B1299, IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti- MNAT1 antibody, ASA-B1299, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue