More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrix metallopeptidase 16(membrane-inserted) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16(582-598aa, YTVFQFKRKGTPRHILY), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MMP16 |
Protein | Matrix metalloproteinase-16(MMP-16) |
Uniprot ID | P51512 |
Function | Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. |
Tissue Specificity | Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary. |
Sub-cellular localization | Isoform Long: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Note: Localized at the cell surface of melanoma cells. |
Sequence Similarities | Belongs to the peptidase M10A family. |
Aliases | C8orf57 antibody|DKFZp761D112 antibody|Matrix metallopeptidase 16 membrane inserted antibody|Matrix metalloproteinase 16 antibody|Matrix metalloproteinase-16 antibody|Membrane type 3 matrix metalloproteinase antibody|Membrane type matrix metalloproteinase 3 antibody|Membrane-type matrix metalloproteinase 3 antibody|Membrane-type-3 matrix metalloproteinase antibody|MMP 16 antibody|MMP X2 antibody|MMP-16 antibody|MMP-X2 antibody|MMP16 antibody|MMP16_HUMAN antibody|MMPX2 antibody|MT MMP2 antibody|MT MMP3 antibody|MT-MMP 3 antibody|MT-MMP2 antibody|MT-MMP3 antibody|MT3 MMP antibody|MT3-MMP antibody|MT3MMP antibody|MTMMP2 antibody|MTMMP3 antibody|Putative transmembrane protein C8orf57 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MMP16 antibody, ASA-B1289, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-MMP16 antibody, ASA-B1289, IHC(P)
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue
