More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrix metallopeptidase 14(membrane-inserted) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14(567-582aa TPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MMP14 |
Protein | Matrix metalloproteinase-14 |
Uniprot ID | P50281 |
Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2. |
Tissue Specificity | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. |
Sub-cellular localization | Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. |
Sequence Similarities | Belongs to the peptidase M10A family. |
Aliases | Matrix metallopeptidase 14(membrane inserted) antibody|Matrix metalloproteinase 14 antibody|Matrix metalloproteinase-14 antibody|Membrane type 1 matrix metalloproteinase antibody|Membrane type 1 metalloprotease antibody|Membrane type matrix metalloproteinase 1 antibody|Membrane-type matrix metalloproteinase 1 antibody|Membrane-type-1 matrix metalloproteinase antibody|MMP 14 antibody|MMP X1 antibody|MMP-14 antibody|MMP-X1 antibody|Mmp14 antibody|MMP14_HUMAN antibody|MMPX1 antibody|MT MMP 1 antibody|MT-MMP 1 antibody|MT1 MMP antibody|MT1-MMP antibody|MT1MMP antibody|MTMMP 1 antibody|MTMMP1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MMP14 antibody, ASA-B1287, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-MMP14 antibody, ASA-B1287, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-MMP14 antibody, ASA-B1287, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue