More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| macrophage migration inhibitory factor (glycosylation-inhibiting factor) | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MIF |
Protein | Macrophage migration inhibitory factor |
Uniprot ID | P14174 |
Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
Tissue Specificity | |
Sub-cellular localization | Secreted. Cytoplasm. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. |
Sequence Similarities | Belongs to the MIF family. |
Aliases | acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody|GIF antibody|GLIF antibody|Glycosylation inhibiting factor antibody|Glycosylation-inhibiting factor antibody|L-dopachrome isomerase antibody|L-dopachrome tautomerase antibody|Macrophage migration inhibitory factor antibody|Macrophage migration inhibitory factor antibody|MIF antibody|MIF antibody|MIF protein antibody|MIF_HUMAN antibody|MMIF antibody|Phenylpyruvate tautomerase antibody|Phenylpyruvate tautomerase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MIF antibody, ASA-B1276, Western blotting
All lanes: Anti MIF (ASA-B1276) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 12KD
Observed bind size: 12KD
All lanes: Anti MIF (ASA-B1276) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 12KD
Observed bind size: 12KD
Anti- MIF antibody, ASA-B1276, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti- MIF antibody, ASA-B1276, ICC
ICC: JURKAT Cell
ICC: JURKAT Cell